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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification
doi: 10.3389/fcell.2020.618228
Figure Lengend Snippet: Fetuin-A and MGP were decreased in ESRD-Ex. (A) The protein concentration of exosomes in Nor-Ex, ESRD-Ex, and RTR-Ex. (B,C) The content of Fetuin-A and MGP in Nor-Ex, ESRD-Ex, and RTR-Ex were measured by ELISA. (D) Western blot analyses detected the levels of Fetuin-A and MGP protein. n = 3. (E) The CAC total score of Nor, ESRD, and RTR patients were calculated by a multilayer spiral CT. n = 8. The representative images were shown. The arrows indicated the calcified part of different branches of the coronary artery. (F,G) The spearman analysis showed the correlations between the content of plasma exosomes Fetuin-A and MGP with CAC total score, respectively. n = 24. *** p < 0.001, ** p < 0.01, * p < 0.05, compared with Nor. ## p < 0.01, # p < 0.05, compared with ESRD. ns, not significant; Nor-Ex, the plasma exosomes derived from normal health control; ESRD-Ex, the plasma exosomes derived from ESRD patients; RTR-Ex, the plasma exosomes derived from renal transplant recipients; CAC, coronary artery calcification.
Article Snippet: Primary antibodies including CD9 (ab92726, 1:1,000, Abcam), CD63 (ab68418, 1:1,000, Abcam), CD81 (ab79559, 1:1,000, Abcam), Runx2 (ab76956; 1:1,000, Abcam), Fetuin-A (16571-1-AP, 1:1,000, Proteintech),
Techniques: Protein Concentration, Enzyme-linked Immunosorbent Assay, Western Blot, Clinical Proteomics, Derivative Assay, Control